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. 2013 Aug 26;41(21):9680–9687. doi: 10.1093/nar/gkt763

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — iss genes contribute to Mmi1-mediated mRNA elimination. (A) Growth profiles of deletion mutants of each iss gene. The 10-fold serial dilutions of the wild-type (JY450), pab2Δ (JV833), iss3Δ (JV832), iss4Δ (JV835), iss6Δ (JT958), iss9Δ (JV967) and iss10Δ (JV969) strains were spotted on YE media and incubated at the indicated temperatures. (B) Expression of mei4 and ssm4 mRNAs in deletion mutants of iss genes under vegetative growth conditions. Transcripts of mei4 and ssm4 were analyzed by northern blot analysis in the wild-type (JY450), mmi1-48 (JT221), iss3Δ (JT959), iss4Δ (JV835), iss6Δ (JT958), iss9Δ (JV967) and iss10Δ (JV969) strains. rRNAs stained with ethidium bromide are shown in the bottom of the panel as loading controls. (C) Expression of Mmi1-target genes was analyzed by quantitative RT-PCR in the same conditions as (B). Transcripts of mei4, ssm4, rec8 and spo5 were quantified and normalized to actin. Results represent the mean ± standard deviation from three independent samples. *P < 0.05; **P < 0.01 compared with the wild-type strain (Student’s t-test).