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. 2013 Aug 26;41(21):9680–9687. doi: 10.1093/nar/gkt763

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Iss10 is required for the proper localization of Red1 to Mmi1 foci. (A) Localization of Red1 in iss10Δ cells. Wild-type (JT961) and iss10Δ (JT962) cells expressing Red1-GFP and CFP-Mmi1 from the respective endogenous promoters were examined by fluorescence microscopy. Images of the nuclear region are shown. Merged images are shown in the right panels: green, Red1-GFP; red, CFP-Mmi1. (B) Localization of Iss10 in red1Δ cells. Wild-type (JT963) and red1Δ (JT964) cells expressing Iss10-GFP and CFP-Mmi1 were examined. Merged images: green, Iss10-GFP; red, CFP-Mmi1. (C) Localization of overexpressed Red1 in iss10Δ cells. Wild-type (JT965) and iss10Δ (JT966) cells expressing Red1-YFP from an expression plasmid and CFP-Mmi1 from an endogenous promoter were examined. Merged images: green, Red1-YFP; red, CFP-Mmi1. (D) Localization of overexpressed Iss10 in red1Δ cells. Wild-type (JT965) and red1Δ (JT967) cells expressing Iss10-GFP from an expression plasmid and CFP-Mmi1 from an endogenous promoter were examined. Merged images: green, Iss10-GFP; red, CFP-Mmi1. Bars, 2 µm.