Table 1.

Primers used for UGA-to-UGG substitutions

This table lists the primers used for introduction of mutations at the codon corresponding to Sec within the open reading frame of rat TrxR1, with or without a downstream SECIS element (see scheme in Figure 1). The nucleotides in bold denote the codon change, whereas the shaded nucleotides introduce restriction cleavage sites for cloning. The R_1 primer was used as a reverse primer in all PCR reactions.

N, represents G, A, U or C.