Figure 2.

Phylogenetic analysis of SUP05/Arctic96BD-19 16S rRNA gene clones recovered from the Inferno vent plume and background seawater (1000 m). Sequences obtained in this study were aligned with those used to generate a robust maximum likelihood reference tree that contained nearly complete (1300 bp), high-quality, nonredundant reference sequences from cultures (*), environment clones and symbionts. Bootstrap values ⩾70 (1000 replicates) are reported above the nodes, except where indicated for lower values (^). Methylococcus capsulatus was selected as an outgroup. SUP05/Arctic96BD-19 subclade designations (black bars) are based on the study by Walsh et al. (2009). Symbiont and free-living (open ocean, coastal and vent) designations (right) indicate the environment from which each rRNA sequence was obtained.