Table 1. 16S rRNA gene clones recovered from the Inferno hydrothermal vent plume.
| Taxonomic assignment | Predicted TRF (bp) | Observed TRF (bp) | Number of clones |
Pearson and Kendall correlations |
|
|---|---|---|---|---|---|
| NMS axis 1 | NMS axis 2 | ||||
| SUP05 | 193 | 194 | 1 | 0.220 | −0.356 |
| Gallionellaceae | 221 | 220 | 2 | 0.496 | 0.006 |
| Methylobacter sp. | 255 | 254 | 2 | −0.110 | 0.204 |
| Methylobacter sp. | 257 | 256 | 2 | −0.018 | −0.042 |
| Gamma-proteobacteria | 259 | 257 | 1 | −0.074 | 0.078 |
| SAR11 | 293 | 291 | 2 | 0.079 | 0.665 |
| Methylomonas sp. | 322 | 322 | 1 | 0.229 | −0.124 |
| SUP05/Arctic96BD-19 | 323 | 324 | 3 | −0.085 | 0.329 |
| Arctic96BD-19 | 327 | 323 | 1 | −0.085 | 0.329 |
| SAR324 | 406 | 404 | 3 | −0.152 | −0.855 |
| SUP05 | 408 | 406 | 1 | 0.044 | 0.267 |
| SUP05 | 410 | 408 | 28 | 0.761 | 0.143 |
Abbreviations: NMS, nonmetric multidimensional scaling; rRNA, ribosomal RNA; TRF, terminal restriction fragment.
Clones were sequenced, grouped by predicted TRFs and annotations and summed to determine the number of clones recovered for each lineage (total observed). Representative clones were selected for each group, amplified, restricted and matched to TRFs observed in environmental samples. TRF correlations with NMS axes 1 and 2 correspond to fragments observed in the environmental TRFLP profiles.