Table 1. Origin of the 10 new vampyrellid strains isolated in this study.

Isolate	Collection date	Geographic origin	Habitat	Sample type	Phylogenetic positiona
NVam1 (Penardia sp.)	Spring 2010	North Carolina, USA	Brackish	Muddy sediment, Cape Fear River, near the estuary	Clade C, subclade P
CAraX	Spring 2009	California, USA	Marine	Sandy sediment, San Francisco Ocean Beach	Clade B, lineage B5
MVa1x (Thalassomyxa sp.)	Summer 2011	Majorca, Spain	Marine	Diatom-rich coastal sandy sediment	Clade B, subclade T
KibAr	Autumn 2007	Cumbria, UKb	Marine	Coastal sediment and rock scrapings, Walney Island	Clade B, subclade T
En42C	Autumn 2007	Cumbria, UKb	Marine	Coastal sediment and rock scrapings, Walney Island	Clade B, subclade T
V1ld4 c	Autumn 2010	Cumbria, UKb	Marine	Coastal sediment and rock scrapings, Walney Island	Clade B, subclade T
V2ld4	Autumn 2010	Cumbria, UKb	Marine	Coastal sediment and rock scrapings, Walney Island	Clade B, subclade T
V1ld9 d	Autumn 2010	Cumbria, UKb	Marine	Coastal sediment and rock scrapings, Walney Island	Clade B, subclade T
WaAra	Spring 2008	Wales, UK	Freshwater	Mixture of moss and lichens from a garden in Gregynog	Clade A, Leptophryidae
BAra1	Summer 2009	Amazon basin, Brazil	Freshwater	Mixture of aquatic plants from a small Rio Negro tributary	Clade A, Leptophryidae

^aSee Figures 4 and 5.

^bSamples collected in the same location.

^cIsolates V1ld4 and KibAr have identical small-subunit ribosomal DNA gene sequences.

^dIsolates V1ld9 and En42C have <0.3% overall small-subunit ribosomal DNA gene sequence divergence.