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. 1979 Apr;76(4):1828–1832. doi: 10.1073/pnas.76.4.1828

Solubilized and insolubilized bone morphogenetic protein.

M R Urist, A Mikulski, A Lietze
PMCID: PMC383485  PMID: 221908

Abstract

A bone morphogenetic protein (BMP) obtained in solution by digestion of demineralized rabbit cortical bone matrix with bacterial collagenase retains its biologically active conformation in a neutral salt/ethylene glycol mixture. BMP may be insolubilized by coprecipitation with calcium phosphate and resolubilized by chemical extraction with a neutral salt in the same solvent mixture. Upon concanavalin A-Sepharose chromatography, BMP is bound by hydrophobic interaction and carbohydrate recognition and is recovered by elution with either alpha-methyl mannoside or ethylene glycol solvent mixture. Implants of both eluates and the extracts of the coprecipitate in double-walled diffusion chambers induce transmembrane bone morphogenesis. BMP is not species specific; rabbit BMP induces new bone formation in the rat. The present observations indicate that BMP is a glycoprotein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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