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. 2013 Jul 25;7(12):2400–2410. doi: 10.1038/ismej.2013.123

Figure 4.

Figure 4

Effect of lysine supplementation on the S. pomeroyi growth rate in high-salt medium. Bacterial cultures were grown for 36 h in 12.5% MB medium containing the indicated salt concentrations: (a) no salt; (b) 100 mM NaCl, (c) 200 mM NaCl, (d) 400 mM NaCl, in the presence or absence of 5 mM lysine. Growth curves were analyzed for statistic significance based on comparison of log phases slopes using covariance analysis. (a) not significant (P=0.43); (b) significant (P=0.008); (c) significant (P=0.0007). (d) significant (P=0.0008). (e) Relative mRNA levels of lysdh, aasadh, lkr, sdh and p5cr, as determined by quantitative real-time PCR, in bacterial cells grown for 24 h in high salt. The fold changes in mRNA levels were estimated using the ΔΔCT method based on the CT of glyceraldehydes-3-phosphate dehydrogenase as the endogenous control. Changes in the relative mRNA levels were compared using Student's t-test. One and two asterisks represent significant differences at P<0.05 and P<0.1, respectively.