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. 2013 Oct 15;6(6):1723–1728. doi: 10.3892/ol.2013.1630

Figure 1.

Figure 1

PKG II inhibits EGF-induced Y1173 phosphorylation of EGFR. The AGS cells were infected with Ad-LacZ or Ad-PKG II for 24 h and serum starved overnight The cells in the Ad-LacZ+EGF and Ad-PKG II+EGF groups were incubated with 100 ng/ml EGF for 5 min. The cells in the Ad-PKG II+cGMP+EGF groups were incubated with 8-pCPT-cGMP for 1 h and then with 100 ng/ml EGF for 5 min. The cells were harvested and lysed as described in Materials and methods and the cell lysate was subjected to western blotting. The results revealed that the infection with Ad-PKG II caused a marked increase in PKG II expression. EGF treatment induced an increase in Y1173 phosphorylation of EGFR. Infection with Ad-PKG II+cGMP efficiently inhibited the EGF-induced phosphorylation of EGFR. PKG II, cGMP-dependent protein kinase Type II; EGFR, epidermal growth factor receptor.