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. 2013 Oct 4;43(6):1824–1832. doi: 10.3892/ijo.2013.2130

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Copyright © 2013, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Figure 2. — Time- and dose-dependent effects of HSP90 inhibition on neuroendocrine cell viability. (A) BON1, (B) NCI-H727 and (C) GOT1 cells were treated with increasing concentrations (0.1–100 nM) of the HSP90 inhibitor AUY922 or HSP990 for indicated time points (24–144 h). Cell viability based on metabolic activity was measured with Cell Titer 96 kit (Promega; upper panels). Cell proliferation based on DNA content was determined by fluorescence SYBR-Green DNA labelling (lower panels). The mean values ± SD of 3 independently performed experiments in sextuplicates (n=18) are demonstrated. ^*p<0.05 versus untreated control.

Figure 2. — Time- and dose-dependent effects of HSP90 inhibition on neuroendocrine cell viability. (A) BON1, (B) NCI-H727 and (C) GOT1 cells were treated with increasing concentrations (0.1–100 nM) of the HSP90 inhibitor AUY922 or HSP990 for indicated time points (24–144 h). Cell viability based on metabolic activity was measured with Cell Titer 96 kit (Promega; upper panels). Cell proliferation based on DNA content was determined by fluorescence SYBR-Green DNA labelling (lower panels). The mean values ± SD of 3 independently performed experiments in sextuplicates (n=18) are demonstrated. ^*p<0.05 versus untreated control.