Figure 3.

Time-course of cone-specific gene expression. The expression of the cone-specific M-opsin (Opn1mw), S-opsin (Opn1sw), and cone transducin alpha subunit (Gnat2) genes was assessed with quantitative PCR. Total RNA was extracted from the retinas of the wild-type, Rpe65^−/−, Cspg5^−/−, and Cspg5^−/−/Rpe65^−/− mice at ages 2 weeks (w) to 6 months (m). For each time point and genotype, three animals were analyzed in duplicate. Wild-type retinal mRNA expression was arbitrarily set to 1 at 2 weeks. For all panels, fold inductions±standard error of the mean (SEM) are shown. With two-way ANOVA, using factors of time and genotypes, no significant changes in relative mRNA expression between the wild-type and Cspg5^−/− mice, and between Rpe65^−/− and Cspg5^−/−/Rpe65^−/− mice were detected.