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. 2013 Nov 16;19:2312–2320.

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Copyright © 2013 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.

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Stra6 protein expression in the P14 mouse retina. Immunohistochemical analysis was performed with a rabbit polyclonal serum raised against the C-terminus of Stra6 (red), on retinal sections of wild-type (A), Rpe65^−/− (B), Cspg5^−/−, and (C) Cspg5^−/−/Rpe65^−/− (D) mice. Stra6 was predominantly expressed at the basolateral membrane of the retinal pigment epithelium (RPE) in all analyzed genotypes. The nuclei were stained in blue with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI), and the images were merged. As a negative control, a serum of a non-immunized rabbit was used, the nuclei stained with DAPI, and the images merged (E). Abbreviations: retinal pigment epithelium (rpe); photoreceptor outer segments (os); photoreceptor inner segments (is); outer nuclear layer (onl). Scale bar equals 40 μm.