Table 1. Primers for quantitative PCR.
| Gene (mus) | Forward primer (5′-3′) | Reverse primer (5′-3′) | GenBank accession number | Location (nt) |
|---|---|---|---|---|
| Abca4 |
ATCGAGGAGTACTCAGTCAC |
CCTGGTGTGCTTATCTTCTG |
NM_007378 |
6775–7008 |
| Gnat1 |
AGAGGATGCTGAGAAGGATG |
ACTGAATGTTGAGCGTGGTC |
NM_008140 |
104- 315 |
| Gnat2 |
CATCAGTGCTGAGGACAAAG |
GACTTGAACTCTAGGCACTC |
NM_008141 |
93- 294 |
| Impg1 |
AATCGAAAGCTACTCCCTCG |
TTGTAGAGTGATCTGGTGGC |
NM_022016 |
2084–2332 |
| Impg2 |
CTTCTGCTGTCGTCTTCTTC |
CCAATCACCTCTTCACTAGC |
BC048863 |
3544–3769 |
| Lrat |
AGCCTACTGTGGAACAACTG |
CAGACATCATCCACAAGCAG |
NM_023624 |
724- 953 |
| Cspg5 |
GACTGAGAATACCAAGCTGC |
TTGGGTGACATGGAGTTCTG |
NM_013884 |
1500–1700 |
| Opn1mw |
TGAGGATAGCACCCATGCAA |
GGCGCAGCTTCTTGAATCTC |
NM_008106 |
83- 282 |
| Opn1sw |
TGGTCAACAATCGGAACCAC |
AGGGCCAACTTTGCTAGAAG |
NM_007538 |
874–1100 |
| Rbp1 |
AATCGCCAACTTGCTGAAGC |
TCTGCACACACTGGAGTTTG |
X60367 |
2113–2312 |
| Rbp3 |
TACTTCTTTGACGAGGCACC |
CTTGTCACTTCACCGATGAC |
NM_015745 |
3361–3584 |
| Rdh5 |
TACCTCCAGCTATACAGGCC |
ATCCAGGTGAGCACAGCATC |
NM_134006 |
867–1107 |
| Rdh12 |
TCACTCGAGAACTGGCTAAG |
TGACGTTCCACAATCGCTCA |
NM_030017 |
756–1046 |
| Rho |
CTCCATGCTGGCAGCGTACA |
TGCTCATCGGCTTGCAGACC |
NM_145383 |
189- 511 |
| RL8 |
ACTGGACAGTTCGTGTACTG |
GCTTCACTCGAGTCTTCTTG |
NM_012053 |
271- 469 |
| Stra6 | GCTACTCCGAGAAGTATCTG | TTCTGCACAGTAGGCACCAC | AF062476 | 1083–1323 |
The location of the PCR amplification products is indicated with respect to nucleotide numbering of the indicated GenBank accession numbers.