Table 1. Humanization of N-glycans in P. pastoris. Selected studies focusing on the humanization of the N-glycans on P. pastoris derived glycoproteins. Bmt, β-mannosyltransferase; Mns, mannosidase; GnT, β-N-acetylglucosaminyltransferase; UDP-GlcNAc, uridine diphosphate-N-acetylglucosamine; OCH1, outer chain elongation gene 1.

content	references
introduction of α-1,2-Mns, GnTI and an UDP-GlcNAc transporter via a combinatorial genetic library approach in a Δoch1::URA3 strain	13,50
introduction of an UDP-GlcNAc transporter, α-1,2-MnsIA, MnsII, GntI, GntII in a Δoch1::URA3 strain	51
introduction of α-1,2-Mns and GnTI, OCH1 inactivation via a knockin plasmid	10
GlycoSwitch plasmids for OCH1 inactivation and introduction of glycosidase and glycosyltransferase activities to produce complex terminally galactosylated glycoproteins	52
introduction of sialic acid biosynthesis pathway and corresponding transporter and transferase activities to produce complex terminally sialylated glycoproteins	53
elimination of α-Mns resistant glycan structures by inactivation of the activities of Bmt1p, Bmt2p and Bmt3p	54