Table 1.
Reactants and products structures and yields of formation dinucleotide cap analogues.
| Product (No) | ελ260 (M−1cm−1) | Nucleophile (No) | Activated nucleotide (No) | HPLC conv. [%] | Yield a/b [%] |
|---|---|---|---|---|---|
| Ant-m7GpppG (1) | 21700 | Ant-m7GDP (6) | GMP-Im (15) | 92 | 39/34 |
| Mant-m7GpppG (2) | 26800 | Mant-m7GDP (7) | GMP-Im (15) | 81 | 36/17 |
| Mant-m7GppCH2pG (3) | 26400 | Mant-m7GMP (8) | GpCH2p-Im (18) | 63 | 36/12 |
| Mant-m7GpCH2ppG (4) | 24000 | Mant-m7GpCH2p (9) | GMP-Im (15) | 72 | 23/8 |
| Ant-m27,2′-OGpppG (5) | 21600 | Ant-m27,2′-OGMP (10) | GDP-Im (17) | 95 | 83/26 |
a
yield after ion exchange chromatography;
b
yield after additional RP HPLC purification