Table 2.

Spectroscopic properties of cap analogues in phosphate buffer and organic solvents.

Compound	Solution[a]	Absorption		λmax (nm)	Fluorescence
		λmax (nm)	ελmax (M−1cm−1)		QY	lifetime
Ant-m7GpppG (1)	PBS pH = 5.18	334	4080	422.5	0.138	τ1= 4.24 ns α1= 0.3181 τ2= 1.78 ns α2= 0.6819 τAV= 2.56 ns χR2=1.026
	PBS pH = 8.96	334	4040	422.5	0.135	τ1= 4.15 ns α1= 0.3172 τ2= 1.79 ns α2= 0.6828 τAV= 2.54 ns χR2= 0.973
	MeOH	341.5	4800	415	0.453	τ1= 7.30 ns α1= 0.8493 τ2= 3.20 ns α2= 0.1507 τAV= 6.69 ns χR2= 0.983
	EtOH	342.5	4670	413	0.594	τ1= 8.08 ns χR2= 1.195
Ant-m7,2′-OGpppG (5)	PBS pH = 5.18	334	4110	424	0.161	τ1= 4.23 ns α1= 0,3689 τ2= 2.04 ns α2= 0.6311 τAV= 2.85 ns χR2= 0.981
	PBS pH = 8.96	334	4100	424	0.156	τ1= 4.23 ns α1= 0.3903 τ2= 2.10 ns α2= 0.6097 τAV= 2.93 ns χR2=0.954
Mant-m7GpppG (2)	PBS pH = 5.18	361	4670	446	0.236	τ1= 7.11 ns α1= 0.4050 τ2= 2.61 ns α2= 0.5950 τAV= 4.43 ns χR2= 1.101
	PBS pH = 8.96	361	4730	446	0.258	τ1= 7.65 ns α1= 0.4498 τ2= 2.81 ns α2= 0.5502 τAV= 4.99 ns χR2= 1.084
	MeOH	358.5	5880	430	0.439	τ1= 8.05 ns α1= 0.6996 τ2= 5.54 ns α2= 0.3004 τAV= 7.30 ns χR2= 1.036
	EtOH	358.5	5500	430	0.484	τ1= 8.24 ns χR2= 1.219

^aPBS = phosphate buffer 0.067 M