Table 1.

Experimental design for 2D-DIGE analysis

Gel number	Cy3 labeling (50 μg protein)	Cy5 labeling (50 μg protein)	Cy2 labeling (50μg protein)	Number of detected spots	Percentage of matched spot referenced to gel 1
1	FWH1	FKS3	IS	1795	100%
2	FWH2	FKC3	IS	1634	77%
3	FWC1	FKH3	IS	1630	79%
4	FWC2	FKS4	IS	1546	76%
5	FWS1	FKC4	IS	1524	74%
6	FWS2	FKH4	IS	1543	77%
7	FKH1	FWH3	IS	1650	79%
8	FKH2	FWS3	IS	1505	75%
9	FKC1	FWC3	IS	1627	78%
10	FKC2	FWH4	IS	1541	75%
11	FKS1	FWC4	IS	1589	76%
12	FKS2	FWS4	IS	1519	73%
13	MWH1	MKS3	IS	1502	63%
14	MWH2	MKC3	IS	1741	75%
15	MWC1	MKH3	IS	1702	73%
16	MWC2	MKS4	IS	1572	67%
17	MWS1	MKC4	IS	1754	74%
18	MWS2	MKH4	IS	1733	72%
19	MKH1	MWH3	IS	1662	74%
20	MKH2	MWS3	IS	1562	70%
21	MKC1	MWC3	IS	1606	70%
22	MKC2	MWH4	IS	1490	68%
23	MKS1	MWC4	IS	1487	70%
24	MKS2	MWS4	IS	1672	73%

Fifty micrograms of the proteins extracted from each sample were labeled with Cy3 or Cy5 as indicated. Cy2-labeled Internal Standard (IS) was prepared by combining equal portions of the 48 left ventricular samples. Analytical gels of left ventricular proteins from MWS, male wild-type sham-operated mice (n = 4); MWC, male wild-type noncongested mice (n = 4); MWH, male wild-type mice with congestive heart failure (n = 4); FWS, female wild-type sham-operated mice (n = 4); FWC, female wild-type noncongested mice (n = 4); FWH, female wild-type mice with congestive heart failure (n = 4); MKS, male FKBP12.6 overexpressing sham-operated mice (n = 4); MKC, male FKBP12.6 overexpressing noncongested mice (n = 4); MKH, male FKBP12.6 overexpressing mice with congestive heart failure (n = 4); FKS, female FKBP12.6 overexpressing sham-operated mice (n = 4); FKC, female FKBP12.6 overexpressing noncongested mice (n = 4); FKH, female FKBP12.6 overexpressing mice with congestive heart failure (n = 4).