Table 3.
Differentially expressed nuclear proteins identified by Westen blots in MCF7 and C4.12.5 cells.
| Gene | MCF7 | C4.12.5 | Nomenclature |
|---|---|---|---|
| BRCA1 | 1 | 0.3 | Breast and ovarian cancer type I |
| TOPOII | 1 | 0 | Type II topoisomerase |
| IRS-I | 0 | 0 | Insulin receptor substrate-1 |
| PARP | 1 | 2 | Poly ADP ribose polymerase |
| MET | 1 | 0 | Met tyrosine kinase |
| p120 | 1 | 1.39 | Zinc finger protein 120 |
| AR | 1 | 0.64 | Androgen receptor |
| Sp1 | 1 | 0.79 | Trans-acting transcription factor 1 |
| Foxo1 | 1 | 0.5 | Forkhead Box A1 |
| ER | 1 | 0.59 | Estrogen receptor |
| WT1 | 1 | 0.8 | Wilms' tumor suppressor gene-1 |
| KLF6 | 1 | 1.2 | Kruppel-like factor 6 |
| LaminA/C | 1 | 2.4 | Nuclear protein |
| Smad 2 | 1 | 1.12 | Smad 2/3 family |
| Smad 3 | 0 | 2.6 | |
| pSmad2/3 | 1 | 1.13 | |
| c-Myc | 1 | 1.48 | Myelocytomatosis oncogene |
| E2F1 | 1 | 0.88 | E2F transcription factor 1 |
| TCF1 | 1 | 0.717 | T-cell factor 1 |
| TCF3A | 1 | 0.002 | High Mobility Group box transcription factor 3 |
| LEF1 | 1 | 1.15 | Lymphoid enhancer binding factor 1 |
| p53 | 1 | 0.96 | p53-related tumor suppressors |
| p63 | 1 | 1.12 | |
| p73 | 1 | 0.03 | |
| p21 | 1 | 0.91 | Waf1/Cip1 |
| GSK3α | 1 | 1.05 | Glycogen Synthase Kinase-3 |
| c-fos | 1 | 0.92 | c-fos/c-jun family of transcription factors |
| c-jun | 1 | 10 | |
| pc-jun | 1 | 0.5 | |
| Cyclin G | 1 | 0.13 | Regulatory subunits of Cdc2 p34 |
| Cyclin D | 1 | 0 | |
| Histone1 | 1 | 0.74 | Nuclear protein |
A value of 1 was assigned to the level of expression in whole MCF7 cell lysates, normalized to tubulin.