Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jun 9;2(2):1236–1250. doi: 10.3390/cancers2021236

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Characterization of CTCs. CTCs isolated from SCLC patients by either the Veridex CellSearch Platform or isolated by ScreenCell ISET size exclusion. Panel A shows a single CTC, isolated by ISET and stained by H&E, with high nuclear to cytoplasmic ratio, cell size >8 µm (the pore size of membrane) and irregular nuclear shape. Panel B shows a SCLC CTM (black arrow) isolated by ISET alongside a leukocyte positively stained brown for CD45 (white arrow). Panel C demonstrates that the DAPI nuclear staining can identify apoptosis (left panel) and nuclear morphology consistent with mitosis (right panel) of CTCs detected by the CellSearch. Panel D SCLC associated diagnostic biomarkers, NSE and TTF-1 were immunohistochemically stained with white blood cells used as internal negative control to further confirm the identity of ISET isolated CTM and CTCs Panel E shows FISH analysis to detect bcl-2 genetic abnormalities such as amplification and triploidy from SCLC CTCs. Blood samples were collected from SCLC patients undertaking chemotherapy at the Christie Hospital, Manchester UK according to ethically approved protocols.