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MVt-raver1 interactions are permissive for binding to RNA.
EMSA of biotin-labeled vinculin RNA and raver1 proteins in complex with MVt establishes that the metavinculin:raver1 complex can bind to this RNA. Note that RRM2-RRM3 protein failed to bind vinculin RNA (lane 4) and that vinculin RNA binding was impaired by the K93E raver1 mutant (lanes 8, 9).
