Figure 2.

Inhibition of 3T3-L1 adipocyte differentiation by EA. (a) Representative photographs showing the cell monolayers stained with Oil Red O (×100). 3T3-L1 preadipocytes were seeded, cultured until 2 day after confluence, and then differentiated by incubating in the growth medium containing 10% fetal bovine serum in the presence of IBMX, dexamethasone, and insulin (MDI). Either EA or roscovitine was added to the incubation medium at the time inducing cell differentiation with MDI. At day 9, the cells were stained with Oil Red O. (b) Immunoblottings for adipogenic markers. Cell lysates were subjected to immunoblottings for PPARγ or aP2. Immunoblotting for β-actin verified equal loading of proteins. (c and d) Densitometric data are expressed as mean ± SD of 4 independent experiments. *P < 0.05 versus control group (without EA treatment).