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. 2013 Oct 25;2(5):e000238. doi: 10.1161/JAHA.113.000238

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© 2013 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an Open Access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 3. — PERK knockdown alters mineralization and osteoblastic differentiation of MOVAS‐1 cells induced by TNFα. A, Mineralization and B, ALP activity of PERK knockdown MOVAS‐1 cells treated with TNFα (10 ng/mL) for 7 days. C, PERK, phosphorylated eIF2α (p‐eIF2α), phosphorylated ATF4 (p‐ATF4), total ATF4 (ATF4), and CHOP protein expression in PERK knockdown MOVAS‐1 cells treated with TNFα (30 minutes for p‐eIF2α and p‐ATF4 expression, 3 hours for ATF4 expression, and 16 hours for CHOP expression). D, RT‐PCR analysis of XBP‐1 in PERK knockdown MOVAS‐1 cells treated with TNFα for 16 hours. The upper band is the expression of uXBP‐1 and the lower is the expression of sXBP‐1. **P<0.01. ALP indicates alkaline phosphatase; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein; eIF2α, eukaryotic initiation factor 2α; PERK, protein kinase RNA‐like endoplasmic reticulum kinase; RSK2, ribosomal S6 kinase 2; TNFα, tumor necrosis factor‐α; XBP, X‐box binding protein‐1.