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. 2013 Oct 25;2(5):e000317. doi: 10.1161/JAHA.113.000317

Figure 2.

Figure 2.

Effects of TGF‐β on sham and CHF‐derived c‐Kit+ cells. c‐Kit+ cells were isolated from sham or CHF EDCs and treated with 10 ng of TGF‐β1 for 7 days. A, TGF‐β caused morphology changes in both sham and CHF‐derived c‐Kit+ cells typical for EMT. Control cells appeared as semiadherent clusters, whereas TGF‐β‐treated cells became attached and elongated. Scale bar=100 μm. B, qRT‐PCR analyses of EMT‐ and pluripotency‐related gene expressions in TGF‐β treated c‐Kit+ cells from sham and CHF explants. Fold changes were calculated as a ratio of the expression in the TGF‐β‐treated group to the expression in the control group. n=5 per group. *P<0.05, TGF‐β treated vs untreated control. CHF indicates chronic heart failure; E‐cadh, E‐cadherin; EDC, explant‐derived cell; EMT, epithelial to mesenchymal transition; qRT‐PCR, quantitative reverse‐transcription polymerase chain reaction; SMA, α‐smooth muscle actin; TGF‐β, transforming growth factor–β.