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. 2013 Oct 25;2(5):e000386. doi: 10.1161/JAHA.113.000386

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© 2013 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an Open Access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 3. — Binding of N50 peptides to endogenous importins shows variations between different human cell types. Biotinylated N50, cN50.1, and N50M peptides (A) or N50 and N50M peptides (B and C) were added to whole cell lysates from: A, Jurkat T cells; B, HeLa S3 cells; or C, EA.hy926 cells. Peptide/protein complexes were isolated with SA‐coated beads and analyzed by immunoblotting with antibodies against a panel of importins. Immunoblotting for the cellular protein GAPDH was used as a control to detect non‐specific interactions. Detection of endogenous proteins in the whole cell lysate is shown in the first column (lysate). Imp α indicates importin α; SA, streptavidin.