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. 2013 Oct 25;2(5):e000386. doi: 10.1161/JAHA.113.000386

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© 2013 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley Blackwell.

This is an Open Access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 7. — Interaction of endogenous importins in Jurkat T cells with peptides derived from their auto‐inhibitory regions. Biotinylated N50 and N50M peptides, as well as biotinylated peptides representing the auto‐inhibitory regions (AR) of Imp α1, α3, α4, α5, and α7, were added individually to whole cell lysates from Jurkat T cells. The peptide and its interacting proteins were pulled down with SA‐coated beads and analyzed by immunoblotting with antibodies against a panel of importins as in Figure 3. Immunoblotting for the cellular protein GAPDH was used as a control to detect non‐specific interactions. Detection of endogenous proteins in the whole cell lysate is shown in the first column (lysate). Imp α indicates importin α; SA, streptavidin.