Figure 2.

Adiponectin deficiency reduces the number of lymphatic endothelial cells in a mouse model of lymphedema. A, Fluorescence staining of tail tissues with lymphatic endothelial cell marker, LYVE‐1 (green), in WT or APN‐KO mice at day 28 after tail ablation surgery or sham operation (white arrows). Total nuclei were identified by 4′,6‐diamidino‐2‐phenylindole counterstaining (blue). B, Quantitative analysis of LYVE‐1–positive cells in the injured tails in WT or APN‐KO mice at day 28 after tail ablation surgery or sham operation (n=7 in each group). Results are shown as the mean±SE. APN indicates adiponectin; KO, knockout; LYVE‐1, lymphatic vascular endothelial hyaluronan receptor‐1; WT, wild‐type.