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. 2013 Nov 20;8(11):e80901. doi: 10.1371/journal.pone.0080901

Figure 1. Nuclear accumulation of Hog1 is delayed under severe hyperosmotic stress.

Figure 1

A. Scheme of the HOG signaling pathway. Upon hyperosmotic shock a branched cascade mediates dual phosphorylation and activation of Hog1. Phosphorylated Hog1 is then translocated into the nucleus where it associates with different DNA-binding proteins to mediate transcriptional regulation. B. Ratio of Hog1-GFP between nucleus and cytosol as a function of time in wild type cells. At time “0” the medium was adjusted to 400mM and 800mM NaCl, respectively. Data represent values for ca. 60 cells for each condition. C. Confocal time lapse images of the nuclear localization of Hog1 in 400mM and 800mM NaCl. Hog1 nuclear localization is delayed under severe osmotic condition (800mM NaCl). See Figure S2 for control data including Nrd1-mCherry, which marks the nucleus. D. Mean ratio of nuclear versus cytosolic Hog1-GFP of about 60 cells as a function of time upon different stress levels ranging from 100mM to 1,000mM NaCl. Colors symbolize the different salt concentrations and symbol sizes correspond to the standard deviation for each time point as indicated.