Table 2.
Identification of phosphorylated sites on peptides in honeybee venom collected by electrical stimulation
| Protein name | Mass | −10lgP | Phosphorylation site | No. of spectra | Z | Site* | Modified peptides and sites |
|---|---|---|---|---|---|---|---|
| Icarapin-like precursor, 33-46 |
1661.8752 |
39.63 |
S43 |
2 |
2 |
33-46 |
R.KNVDTVLVLPS(+79.97)IER.D |
| Icarapin-like precursor, 202-223 |
2485.0896 |
56.65 |
S205 |
2 |
2 |
202-223 |
R.SVES(+79.97)VEDFDNEIPKNQGDVLTA |
| Phospholipase A-2, 145-160 |
2169.0061 |
44.56 |
T145 |
1 |
4 |
145-160 |
Y.T(+79.97)VDKSKPKVYQWFDLR.K |
| Apamin preproprotein 23-40 | 2170.9033 | 30.85 | T23 | 2 | 4 | 23-40 | V.T(+79.97)PVMPCNCKAPETALCAR.R |
Protein name and mass of the identified proteins searched against the NCBInr database in 2.5. Modified site is the position of the initial and final amino acids of peptide in the protein sequence. Phosphorylated amino acid is labeled with Sp. Site* is the position of the initial and final amino acids of the peptide in the protein sequence. No. of spectra means the number of the identified phosphopeptides’s mass spectra. Z means the charge of identified phosphopeptide. The peptides are accepted when -10lgP > 20 (p < 0.01) as well as FDR <0.1%.