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. Author manuscript; available in PMC: 2013 Nov 20.
Published in final edited form as: Arch Pharm Res. 2010 Mar 30;33(3):10.1007/s12272-010-0313-3. doi: 10.1007/s12272-010-0313-3

Fig. 4.

Fig. 4

Effects of KR62776 on the levels of the cell cyclerelated proteins in HSC-T6 cells. Cell lysate proteins (20 μg/lane) were separated on 10% SDS-polyacrylamide gels and subjected to immunoblot analysis with the specific antibody against p21, p27, GADD45, cyclin D1, cdk2, cyclin B, or GAPDH, followed by incubation with a horseradish peroxi-dase-conjugated secondary antibody. Detection of each antigen was performed using the ECL detection kit. The results shown are representative blots of three independent experiments.