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Secondary structure content of rMEHB calculated from far-UV (260–195 nm) CD curves adjustments in 2 mM MOPS pH 7.0 and 8.0 using the network secondary structure estimator from concentration independent Raussens et al. method (http://perry.freeshell.org/raussens.html) [25].
| Secondary structure (%) | pH 7.0 | pH 8.0 | ||
|---|---|---|---|---|
| 25°C | 95°C | 25°C | 95°C | |
| α-helix | 7.5 | 5.3 | 8.0 | 7.4 |
| β-sheet | 32.7 | 36.7 | 33.1 | 33.1 |
| β-turn | 12.5 | 14.5 | 12.5 | 12.9 |
| Random-coil | 40.9 | 40.5 | 40.8 | 40.9 |
