Table 3.

Matrix effect in PBMC extract (n=3).

Conc (ng/mL)		70% MeOH-tris (mean±SD)	PBMC extract (mean±SD)	ME,%
0.5	Peak area	0.403±0.006	0.498±0.011	123
	Ratio	0.0220±0.0005	0.0255±0.0006	116
1.5	Peak area	1.08±0.04	1.24±0.05	115
	ratio	0.0589±0.0005	0.0642±0.0016	109
40	peak area	28.7±1.5	28.1±0.7	98
	ratio	1.62±0.05	1.39±0.05	86

Note: peak area is expressed as ×10⁴ cps. Ratio is calculated with peak area of drug devided by peak area of IS.