Figure 2.

Acetylshikonin attenuated H₂O₂-induced cell death and apoptosis in both SH-SY5Y and PC12 cells with dose-dependent manner. SH-SY5Y or PC12 cells were incubated with desired concentration of acetylshikonin for 12 hours and then stimulated with H₂O₂ (500 μM) for 4 hours, and the change of cell morphology ((a) SH-SY5Y; (b) PC12), cell viability ((c) SH-SY5Y; (d) PC12), and change of nuclear morphology ((e), SH-SY5Y; (f), PC12) were measured, respectively. Data shown are means ± SEM of results from independent experiments in triplicate. *P < 0.05 compared with control cells; ^# P < 0.05 compared with H₂O₂-stimulated cells.