Table 3.
Levels of CA-125, HE4, and 14-3-3 Z protein determined by ELISA and ECLIA methods
| |
|
|
ELISA |
|
|
ECLIA |
|
ELISA |
|---|---|---|---|---|---|---|---|---|
|
Patient no. |
Chemo |
CA-125 |
HE4 |
ROMA |
CA-125 |
HE4 |
ROMA |
14-3-3 Z |
| Cyle no. | (U/mL) | (p/M) | % | (U/mL) | (p/M) | % | (ng/mL) | |
|
1 |
1 |
23.38 |
95.87 |
26.19 |
105.70 |
79.63 |
46.90 |
38.28 |
| |
2 |
18.09 |
65.94 |
16.60 |
76.82 |
30.69 |
20.57 |
37.95 |
| |
3 |
7.78 |
64.47 |
9.49 |
73.55 |
15.77 |
11.20 |
18.78 |
| |
4 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
| |
5 |
10.71 |
67.85 |
12.26 |
77.76 |
8.58 |
6.5 |
32.01 |
| |
6 |
7.90 |
62.81 |
9.35 |
75.21 |
9 |
6.60 |
39.82 |
|
2 |
1 |
26.93 |
334.49 |
58.99 |
353.50 |
176.10 |
83 |
0 |
| |
2 |
24.28 |
102.01 |
28 |
116.10 |
106.20 |
56 |
0 |
| |
3 |
19.31 |
66.42 |
17.37 |
79.16 |
29.24 |
20.10 |
0 |
| |
4 |
10.16 |
87.74 |
14.97 |
88.92 |
18.22 |
14.30 |
0 |
| |
5 |
3.33 |
81.38 |
6.7 |
88.28 |
15.06 |
12 |
0 |
| |
6 |
0.08 |
85.94 |
0.49 |
87.33 |
13.11 |
10.50 |
0 |
|
3 |
1 |
1.45 |
43.73 |
2 |
83.1 |
52.97 |
32.6 |
0 |
| |
2 |
0 |
49.24 |
0 |
67.49 |
28.04 |
17.70 |
0 |
| |
3 |
3.47 |
52.41 |
4.48 |
69.24 |
23.49 |
15.4 |
0 |
| |
4 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
| |
5 |
0 |
44.09 |
0 |
52.38 |
17.25 |
9.7 |
0 |
| |
6 |
9.12 |
52.37 |
8.68 |
62.76 |
24.63 |
15.10 |
0 |
|
4 |
1 |
0 |
48.51 |
0 |
55.06 |
6.78 |
4 |
1.34 |
| |
2 |
0 |
55.9 |
0 |
58.43 |
6.91 |
4.30 |
1.42 |
| |
3 |
3.63 |
47.48 |
4.18 |
54.72 |
6.71 |
4 |
0.39 |
| |
4 |
2.55 |
45.86 |
3.15 |
53.12 |
6.08 |
3.50 |
0.32 |
| |
5 |
4.35 |
51.67 |
5.16 |
58.04 |
6.59 |
4.10 |
0.26 |
| |
6 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
|
5 |
1 |
9.23 |
101.40 |
15.93 |
133.4 |
14.60 |
15.20 |
0 |
| |
2 |
12.40 |
93.07 |
17.77 |
107.40 |
8.69 |
8.20 |
0 |
| |
3 |
2.78 |
85.49 |
6.21 |
103.30 |
7.08 |
6.50 |
0 |
| |
4 |
14.24 |
69.76 |
15.03 |
75.26 |
6.66 |
4.90 |
0 |
| |
5 |
9.10 |
75.68 |
12.20 |
80.91 |
7.95 |
6.20 |
0 |
| |
6 |
9.73 |
74.47 |
12.55 |
80.21 |
7.89 |
6.10 |
0 |
|
6 |
1 |
153.04 |
904 |
93.53 |
1500 |
701.5 |
98.30 |
0 |
| |
2 |
445.65 |
904 |
96.94 |
1218 |
644.20 |
97.90 |
0 |
| |
3 |
368.55 |
714.78 |
95.57 |
843.10 |
394.10 |
95.50 |
0 |
| |
4 |
249.51 |
541.89 |
92.41 |
540.10 |
243.90 |
90.30 |
0 |
| |
5 |
95.43 |
259.90 |
73.68 |
227.30 |
84.13 |
65.40 |
0 |
| |
6 |
39.94 |
167.57 |
48.43 |
142 |
46.16 |
38.30 |
0 |
|
7 |
1 |
77.86 |
118.41 |
51.49 |
111.90 |
68.66 |
44.10 |
4.36 |
| |
2 |
17.01 |
122.23 |
26.50 |
124.90 |
22.64 |
22.64 |
15.02 |
| |
3 |
9.25 |
120.03 |
18.50 |
127 |
18.20 |
17.80 |
8.28 |
| |
4 |
8 |
107.76 |
15.47 |
111.10 |
16.34 |
15 |
6.38 |
| |
5 |
1.07 |
53.14 |
1.98 |
132.50 |
15.39 |
15.80 |
13.13 |
| |
6 |
9.03 |
202.84 |
27.82 |
186.80 |
26.99 |
30.30 |
5.74 |
|
8 |
1 |
475.23 |
259.74 |
90.07 |
248.50 |
4810 |
99.2 |
0.04 |
| |
2 |
500 |
90.83 |
75.92 |
80.07 |
1265 |
92.7 |
0 |
| |
3 |
189.18 |
67.99 |
53.34 |
70.60 |
642.10 |
85.20 |
0 |
| |
4 |
500 |
904 |
97.18 |
1500 |
5000 |
99.8 |
0 |
| |
5 |
172.63 |
57.11 |
47.22 |
57.21 |
254.10 |
65.30 |
0 |
| |
6 |
402.03 |
62.74 |
64.64 |
57.66 |
256.20 |
65.60 |
0 |
|
9 |
1 |
21.16 |
904 |
77.35 |
1500 |
185.40 |
93.70 |
2.46 |
| |
2 |
29.53 |
170.69 |
43.41 |
176.10 |
61.61 |
49.50 |
1.61 |
| |
3 |
4.97 |
97.08 |
10.38 |
103.70 |
21.29 |
18.10 |
1.32 |
| |
4 |
8.13 |
96.13 |
14.09 |
102.70 |
15.37 |
13.50 |
0 |
| |
5 |
5.90 |
105.57 |
12.56 |
108.90 |
13.93 |
12.80 |
0.13 |
| |
6 |
8.57 |
84.25 |
12.96 |
87.24 |
15.70 |
12.40 |
0.06 |
|
10 |
1 |
500 |
904 |
97.18 |
1500 |
5000 |
99.8 |
0 |
| |
2 |
189.91 |
62.04 |
51.08 |
60.85 |
243.10 |
65.30 |
0 |
| |
3 |
500 |
441.44 |
94.22 |
514.20 |
4789 |
99.5 |
0 |
| |
4 |
500 |
149.23 |
84.07 |
155.70 |
833.50 |
93.10 |
0 |
| |
5 |
84 |
127.48 |
54.97 |
121.70 |
197.90 |
71.60 |
0 |
| |
6 |
29.43 |
90.79 |
28.37 |
92.78 |
54.27 |
35 |
0 |
|
11 |
1 |
7.85 |
904 |
62.12 |
1500 |
117.70 |
90.20 |
0 |
| |
2 |
9.58 |
201.95 |
28.59 |
200.90 |
36.28 |
38.40 |
0 |
| |
3 |
0.93 |
131.04 |
4.42 |
125.90 |
18.88 |
18.30 |
0 |
| |
4 |
3.75 |
105.53 |
9.34 |
104.40 |
13.74 |
12.30 |
0 |
| |
5 |
2.65 |
107.64 |
7.54 |
107.80 |
13.41 |
12.30 |
0 |
| |
6 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
|
12 |
1 |
126.02 |
397.04 |
84.2 |
404.30 |
1772 |
98.30 |
0.38 |
| |
2 |
500 |
365.72 |
93.07 |
323.20 |
1517 |
97.70 |
0 |
| |
3 |
192.79 |
273.67 |
83.2 |
258.90 |
761.40 |
94.70 |
0.09 |
| |
4 |
178.79 |
223.37 |
79.09 |
192.20 |
360.10 |
86.80 |
0 |
| |
5 |
121.86 |
168.58 |
68.17 |
169 |
265.70 |
81.30 |
0 |
| |
6 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
|
13 |
1 |
500 |
904 |
97.18 |
1500 |
5000 |
99.8 |
0 |
| |
2 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
| |
3 |
500 |
904 |
97.18 |
1500 |
5000 |
99.8 |
0 |
| |
4 |
500 |
904 |
97.18 |
853.90 |
3241 |
99.50 |
0 |
| |
5 |
120.65 |
355.03 |
82.16 |
349.30 |
171.90 |
82.5 |
0 |
| 6 | NA | NA | NA | NA | NA | NA | NA |
ROMA, risk of malignancy algorithm.
NA, not available - when serum samples were too hemolyzed and measurements could not be made.