Table 2. Sample quality control and RNA-seq outcome.
Sample ID | OD260/280 | RIN † | Total Bases | Total Reads | Mapped Reads |
---|---|---|---|---|---|
1-Control | 2.14 | 9.1 | 2,866,775,400 | 28,667,754 | 37.45% |
2-Control | 2.08 | 8.8 | 3,554,260,200 | 35,542,602 | 70.05% |
3-Control | 2.13 | 9.2 | 3,075,605,600 | 30,756,056 | 66.74% |
4-Null | 2.14 | 9.8 | 3,579,700,400 | 35,797,004 | 28.34% |
5-Null | 2.08 | 9.5 | 3,317,503,200 | 33,175,032 | 33.81% |
6-Null | 2.12 | 9.4 | 4,029,281,000 | 40,292,810 | 40.31% |
High RNA integrity number (RIN) scores (7-10) and a narrow distribution of scores (1-1.5)
from an Agilent Bioanalyzer indicated high RNA sample quality.