Figure 2.
Response of osteoblasts derived from 1-month, 3-month, and 11-month-old rats to Ti surface roughness and hydrophilicity and treatment with 1α,25(OH)2D3. Cells were cultured on TCPS, PT, SLA, and modSLA Ti surfaces with or without 10−7M 1α,25(OH)2D3. (A,D) Cell number, (B,E) alkaline phosphatase specific activity in the cell lysate, and (C,F) osteocalcin levels were determined. Values presented are mean ± SEM of six independent cultures. *p<0.05 vs. TCPS, ^p<0.05 vs. 1-month-old rats.