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. Author manuscript; available in PMC: 2013 Nov 21.
Published in final edited form as: Chem Commun (Camb). 2010 Sep 28;46(43):10.1039/c0cc03141k. doi: 10.1039/c0cc03141k

Fig. 2.

Fig. 2

Synthesis of the l-proinsulin polypeptide chain. (a) LC-MS analytical data (λ = 210 nm) for the first ligation between l-proinsulin-(Thz19-Cys71)-αCOSR and l-proinsulin(Cys72-Asn86), followed by conversion of Thz- to Cys-. (b) LC-MS analytical data for the second ligation between l-proinsulin(Phe1-Val18)-αCOSR and l-proinsulin(Cys19-Asn86); the times shown refer to overall elapsed times for the synthesis. The chromatographic separations were performed using a linear gradient (9%–53%) of buffer B in buffer A over 22 min (buffer A = 0.1% TFA in water, buffer B = 0.08% TFA in acetonitrile).