Figure 2.
Frequency of IFNγ-producing cells in response to pandemic influenza peptides. Pre- and post-vaccination PBMCs were stimulated with 10 μg/ml of pandemic H1N1 peptides 1 and 2, a common H1N1/H3N2 sequence, the seasonal H1N1 and H3N2 peptides (a-e), the whole HA protein (f), a mixture of all the peptides (g) or PHA (h) for 48 h. Cells producing IFN-γ were enumerated using an ex-vivo ELISPOT assay. The results are expressed as the number of SFCs per 106 PBMCs. Insets depict the percentage of subjects with a positive IFN-γ response pre- (dark) and post-(light) vaccination.