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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Cytokine. 2013 Sep 8;64(2):10.1016/j.cyto.2013.08.005. doi: 10.1016/j.cyto.2013.08.005

Figure 1.

Figure 1

NE and ATP induce IL-6 secretion by HMEC-1 cells synergistically. Supernatants were collected after 24 hrs and analyzed by ELISA. (A) Cells treated with 10 µM ATP or each indicated NE concentration alone significantly increased IL-6 (p<0.001) versus DM. Cells treated with 10 uM ATP and each NE concentration induced IL-6 release synergistically. (B) Cells treated with 50 µM ATP or each indicated NE concentration alone significantly increased IL-6 (p<0.001) versus DM. Cells treated with 50 µM ATP and each NE concentration resulted in a synergistic release of IL-6. (C) Cells treated with 100 µM ATP or each NE concentration alone significantly increased IL-6 (p<0.001) compared to DM. Treatment of cells with 100 µM ATP and 10−5 M NE or 10−6 M NE resulted in synergistic induction of IL-6. A representative experiment of 3 (A), 3 (B) and 2 (C) is shown in each graph; results are the mean +/− SD of assays performed in triplicate. ***p<0.001 vs. additive effect of each agent alone.