Figure 3.
Cytotoxicity (A, B) or mutagenicity (C, D) compared to levels of O6-mG detected in CHOpcDNA3 (A, C) and CHOAGT cells (B, D) immediately after a 1 h treatment with NMUr, AMMN or NNK-4-OAc. Levels of O6-mG are averages of three replicates. Cytotoxicity and mutagenicity data are averages from 3–4 separate experiments performed with duplicates or triplicates. Error bars represent standard deviation. The lines were generated by connecting the data points. *Significantly different from NNK-4-OAc, p = 4 × 10−4, and NMUr, p = 4 × 10−9. **Significantly different from NNK-4-OAc, p = 0.035. ***Significantly different from NMUr and NNK-4-OAc.