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. 2013 Nov 20;8(11):e80943. doi: 10.1371/journal.pone.0080943

Table 5. Summary of ORFs encoded by the O-antigen biosynthesis gene cluster of RAh strain AL06-06.

ORF ID Nucleotide positions in Accession no. KC999971 Predicted function Gene Nearest Neighbor %Identity Accession Number
ORFu 1..1719 lipid A core - O-antigen ligase waaL Aeromonas hydrophila subsp. hydrophila ATCC 7966 97 YP_857377
ORF1 1814..2875 O-antigen chain length determinant protein wzzC Aeromonas caviae Ae398 69 ZP_08521419
ORF2 2948..3487 dTDP-4-dehydrorhamnose 3,5-epimerase rmlC Halomonas sp. TD01 57 ZP_08635572
ORF3 3592..4479 glucose-1-phosphate thymidylyltransferase 1 rmlA Aeromonas veronii B565 92 YP_004392190
ORF4 4591..5478 dTDP-4-dehydrorhamnose reductase rmlD Aeromonas hydrophila subsp. hydrophila ATCC 7966 97 YP_857411
ORF5 5478..6566 dTDP-glucose-4, 6-dehydratase rmlB Aeromonas hydrophila 98 AAM22544
ORF6 6761..8722 epimerase/dehydratase family WbfY-like protein wbgZ Aeromonas caviae Ae398 96 ZP_08521420
ORF7 8781..9335 lipid carrier : UDP-N-acetylgalactosaminyltransferase wbtB Aeromonas veronii B565 96 YP_004393428
ORF8 9338..10078 UDP-glucose 4-epimerase galE Vibrio metschnikovii CIP 69.14 77 ZP_05883342
ORF9 10301..11422 glycosyl transferase, group 1 family protein wbxK Shewanella oneidensis MR-1 59 NP_718730
ORF10 11419..13353 asparagine synthetase, glutamine-hydrolyzing asnD Shewanella oneidensis MR-1 74 NP_718731
ORF11 13380..14491 glycosyl transferase, group 1 wbxL Vibrio ichthyoenteri ATCC 700023 46 ZP_08744363
ORF12 14505..15575 group 1 glycosyl transferase wbxM Achromobacter piechaudii ATCC 43553 35 ZP_06689986
ORF13 15572..16861 virulence factor MVIN family protein mviN Burkholderia ubonensis Bu 39 ZP_02383242
ORF14 16858..18057 Cna B domain-containing protein cnaB Flavobacterium sp. F52 33 ZP_10479786
ORF15 18286..19314 UDP-GlcNAc 4-epimerase wbgU Shewanella oneidensis MR-1 81 NP_718745
ORF16 19417..20697 UDP-glucose dehydrogenase wbpO Vibrio vulnificus MO6-24/O 88 YP_004190001
ORF17 21479..21766 dTDP-D-glucose-4,6-dehydratase rmlB Aeromonas hydrophila 90 AAM74474
ORFd 22510..23925 outer membrane protein OprM oprM Aeromonas hydrophila 99 AAM22559