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. Author manuscript; available in PMC: 2013 Nov 21.
Published in final edited form as: Differentiation. 2012 Jun 19;84(2):10.1016/j.diff.2012.04.007. doi: 10.1016/j.diff.2012.04.007

Fig. 2.

Fig. 2

Tbx6 overexpression does not promote cardiac myocyte differentiation. (A) Tbx6 levels in Tbx6 transfectants. P19CL6-MLC-2v-GFP cells were stably transfected with Tbx6 or empty vector or left untransfected. Tbx6 levels were assessed by Western blot. Empty denotes P19CL6-MLC-2v-GFP cells stably transfected with empty vector. Similar results were obtained in two independent experiments. (B) Tbx6 overexpression is not sufficient to induce cardiac myocyte differentiation in the absence of DMSO and does not alter the magnitude of DMSO-induced differentiation. P19CL6-MLC-2v-GFP cells stably transfected with empty vector or Tbx6 were cultured with or without DMSO for 10 days. GFP levels were assessed by Western blot and quantified by densitometry. GFP expression, driven by the striated muscle-specific MLC-2v promoter, serves as a marker of striated muscle differentiation. Graph shows abundance (mean ± SD) of GFP protein normalized to that of GAPDH for three independent experiments (P NS for Tbx6 transfectants vs. empty vector transfectants treated with DMSO). Spontaneous beating was scored using microscopy.