Fig. 5.
Tbx6 overexpression inhibits RA-induced glial and neuronal differentiation. P19CL6-MLC-2v-GFP cells stably transfected with empty vector or Tbx6 were cultured with or without RA for 4 days and harvested on day 8. Levels of GFAP (glial marker) and MAP2 (neuronal marker) were assessed by Western blot. Mean ± SD of protein levels from densitometric analysis are as indicated. Data from three independent experiments (*P < 0.01 for Tbx6 transfectants vs. empty vector transfectants treated with RA).