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. Author manuscript; available in PMC: 2013 Nov 21.
Published in final edited form as: Differentiation. 2012 Jun 19;84(2):10.1016/j.diff.2012.04.007. doi: 10.1016/j.diff.2012.04.007

Fig. 6.

Fig. 6

Tbx6 knockdown augments RA-induced glial and neuronal differentiation. (A) Tbx6 knockdown decreases Tbx6 mRNA levels. P19CL6-MLC-2v-GFP cells stably expressing scrambled shRNA or Tbx6 shRNA were cultured with or without RA for 4 days and Tbx6 mRNA abundance assessed by qRT-PCR. Quantitative data from three independent experiments. (B) Effect of Tbx6 knockdown on RA-induced GFAP and MAP2 expression. Scrambled shRNA and Tbx6 shRNA stable transfectants were cultured with or without RA for 4 days and harvested on day 8. Levels of GFAP (glial marker) and MAP2 (neuronal marker) were assessed by Western blot. Mean ± SD of protein levels from densitometric analysis are indicated. Data from three independent experiments (*P < 0.01).