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. Author manuscript; available in PMC: 2014 May 21.
Published in final edited form as: Biochemistry. 2013 May 7;52(20):10.1021/bi301616c. doi: 10.1021/bi301616c

Figure 2.

Figure 2

Active site titration. (A) The WT human Hint1 (1, 2, 3, 4, 5μM) was mixed with AIPA (10 μM) in stopped flow cell and the time course of IPA production was monitored at 360nm. (B) The amplitude of “burst” vs. human Hint1 monomer concentration was plotted. A slope of 0.77 with R2 =0.955 indicated that 77% of the active site catalyzes the reaction in the first catalytic cycle.