Table 1. Pre-steady state Kinetics of hHint1 using AIPA or TpAd as substrate in HEPES buffer (20mM HEPES, 1mM MgCl2, pH 7.2) at 25°C.
| AIPA | TpAd | |||||
|---|---|---|---|---|---|---|
|
| ||||||
| k2 (s−1) | K adenylyl (μM) | k2/Kadenylyl (M−1s−1) | k2 (s−1) | Kadenylyl (μM) | k2/Kmadenylyl (M−1s−1) | |
| WT | 443 ± 38 | 5 ± 1 | (8.8 ± 2.8) × 107 | 203 ± 13 | 13 ± 2 | (1.6 ± 0.4) × 107 |
| H114A | 851 ± 45 | 35 ± 5 | (2.4 ± 0.5) × 107 | 34 ± 1 | 57 ± 7 | (0.6 ± 0.1) × 106 |
| H110A | 965 ± 83 | 15 ± 4 | (6.4 ± 2.0) × 107 | 29 ± 1 | 12 ± 2 | (2.5 ± 0.4) × 106 |
| H51A | 313 ± 12 | 17 ± 2 | (1.8 ± 0.3) × 107 | 23 ± 1 | 27 ± 2 | (0.9 ± 0.1) × 106 |