Figure 5. DNA content and metabolic activity of KSCs and MSCs in the presence of kidney region-specific solubilized ECM.
A KSCs cultured on tissue culture plastic in the presence of solubilized whole organ or kidney region-specific ECM for 48hrs. B DNA quantification of KSCs revealed less DNA in the presence of solubilized papilla ECM than in the presence of solubilized ECM from cortex, medulla, or whole kidney. C KSCs cultured in the presence of solubilized papilla ECM were significantly more metabolically active than KSCs cultured in the presence of solubilized ECM from cortex, medulla, or whole kidney. D MSCs cultured on tissue culture plastic in the presence of solubilized whole kidney and kidney region ECM for 48hrs. E DNA quantification revealed no significant differences between MSCs cultured in the presence of whole organ or kidney region solubilized ECM. F Metabolic activity of MSCs showed no differences between MSCs cultured in the presence of whole organ or kidney region solubilized ECM. * p < 0.05.