Figure 7. BPI to confirm the protective efficacy of a vaccine based on heat-killed bacteria.

Mice were immunized with either PBS or with heat-killed S. pyogenes strain H347, and then challenged with intranasal H347::lux. The mice were imaged for 4 days post infection using an IVIS 100 system (A). Total flux from mice in each group was compared over the time course (B, Repeated Measures Two way ANOVA with Bonferroni post-test * p<0.05). Colony counts were obtained from dissections performed on the final day of infection (C, Mann-Whitney, * p<0.05). Serum antibody responses against S. pyogenes from tail bleeds taken before infection were measured by ELISA (D). Error bars indicate mean and SD.