Figure 1.

CB₂ receptor is expressed in mouse penis. Immunostaining and western blotting analysis was used to detect CB₂ receptor on penis from wild type and ApoE^−/− mice. (a) CB₂ was strongly colocalized with α-actin (smooth muscle cells marker) as well as PECAM (endothelial cell marker) in the corpus cavernosum and dorsal vessels of wild type and ApoE^−/− mice. The graph shows representative images obtained from 8 different animals. (b) CB₂ in the mouse penis was confirmed by western blotting which revealed a specific single band at the expected molecular weight (approximately 45 kDa). The graph shows a representative gel of western blotting showing the expression of CB₂ in penis from wild type and ApoE^−/− mice from 4 independent experiments.