Figure 3.

CB₂ activation reduces NADPH oxidase and increases eNOS protein expression. ((a)-(b)) JWH-133 treatment decreased p47 levels into the penis of ApoE^−/− mice. (a) Quantification of the Western blotting data. (b) Representative gel. Data were normalized using β-tubulin. ((c)-(d)) Three weeks of JWH-133 treatment increased eNOS protein levels into ApoE^−/− mice penis. (c) Quantification of the western blotting data. (d) Representative gel. Data were normalized using β-tubulin. *P < 0.05 (One-way ANOVA followed by Bonferroni post-test). Each column represents the mean ± SEM of relative protein expression in arbitrary unity (A.U.) from 3 independent experiments. ((e)-(f)) The measurement of the NO stable metabolites, nitrite and nitrate, was determined by Griess assay. JWH-133 treatment significantly increased nitrite and nitrate levels, in serum from ApoE^−/− mice. Each column represents the mean ± SEM (n = 8) of serum nitrite and nitrate levels (μmol/L).