Table 2.

S . cerevisiae strains used in this study

Strain	Genotype, description	Reference
S288c	MATa (prototrophic)	Mortimer and Johnston [46]
W303-1A	MATa (prototrophic)	Thomas and Rothstein [47]
CEN.PK113-7D	MATa (prototrophic)	van Dijken et al. [48]
CEN.PK113-1A	MATα (prototrophic)	P Kötter (Euroscarf)
CBS 6412	Wild-type, diploid	CBS strain collection
CBS 6412-13A	MATa, haploid segregant of CBS 6412	This study
CBS 6412-13A gut1∆	MATa gut1::loxP-kanMX-loxP	This study
CBS 6412-13A gut2∆	MATa gut2::loxP-ble-loxP	This study
CBS 6412-13A stl1∆	MATa stl1::loxP-ble-loxP	This study
CEN.PK113-1A gut1∆	MATα gut1::loxP-kanMX-loxP	This study
Hybrid I	MATa/α; cross of CBS 6412-13A and CEN.PK113-1A	This study
Hybrid II	MATa/α; cross of CBS 6412-13A and CEN.PK113-1A gut1∆	This study
Hybrid III	MATa/α; cross of CBS 6412-13A gut1∆ and CEN.PK113-1A	This study
Hybrid IV	MATa/α; cross of CBS 6412-13A gut1∆ and CEN.PK113-1A gut1∆	This study

The 52 S. cerevisiae strains used in the initial screening for glycerol growth are summarized in Additional file 1. Euroscarf, European Saccharomyces cerevisiae archive for functional analysis.